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| Registros recuperados: 12 | |
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| Cazedey,Edith Cristina Laignier; Carvalho,Flávia Chiva; Fiorentino,Flávia Angélica Másquio; Gremião,Maria Palmira Daflon; Salgado,Hérida Regina Nunes. |
| Tests in animals are used as models in toxicological and investigative studies. However, such tests have been considered inhumane because they can cause pain and suffering to experimental animals, while these methods can often be subjective. Protests calling for animal protection have questioned the effectiveness of in vivo tests and suggest the introduction of alternative, in vitro methods. International organizations, such as the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), the National Institute of Health (NIH), the Organization for Economic Co-operation and Development (OECD), that regulate and develop new alternative animal models, have indicated the running of preliminary assays and execution of sequential... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal experimentation/alternative methods in vitro; Experiments/in vitro methods; Corrositex®/in vitro method/experiments; BCOP/in vitro method/experiments; HET-CAM/in vitro method/experiments. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400021 |
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| Oliveira,Lara Alexandre de; Souza-Moreira,Tatiana Maria de; Cefali,Letícia Caramori; Chiari,Bruna Galdorfini; Corrêa,Marcos Antonio; Isaac,Vera Lucia Borges; Salgado,Hérida Regina Nunes; Pietro,Rosemeire Cristina Linhari Rodrigues. |
| The leaves of the Brazilian species Plinia cauliflora were used to obtain active hydroalcoholic extract and fractions enabling the development of efficient antiseptic pharmaceutical formulations. A chemical composition of 70% ethanol extract, aqueous and ethyl acetate fractions was analyzed by thin-layer chromatography and for phenol content. Antimicrobial activity was tested against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Lactobacillus acidophilus and Candida albicans by the agar diffusion method and the minimum inhibitory concentration was assayed by broth microdilution. Extract microbiological quality was tested to avoid contamination in the formulations. A mouthwash and a topical cream containing the extract were developed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antiseptic formulations/design; Plinia cauliflora/antimicrobial activity; Plinia cauliflora/antiseptic activity; Mouthwash; Plant extract; Topical cream. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300010 |
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| Cefali,Letícia Caramori; Souza-Moreira,Tatiana Maria; Corrêa,Marcos Antônio; Salgado,Hérida Regina Nunes; Isaac,Vera Lucia Borges. |
| Lycopene, a carotenoid and potent antioxidant is found in large quantities in tomatoes. Lycopene combats diseases, such as cardiovascular disease and different types of cancer, including prostate cancer. However, its topical use in emulsion form for the combat of skin aging is under-explored. The aim of the present study was to develop an emulsion containing lycopene extracted from salad tomatoes and evaluate its cytotoxicity, stability, rheological behavior, antioxidant activity and phytocosmetic permeation. The developed cosmetic comprised an oil phase made up of shea derivatives and was evaluated in terms of its physiochemical stability, spreadability, thermal analysis, rheological behavior, microbiological quality, cytotoxicity, antioxidant activity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carotenoid/antioxidant activity; Lycopene/emulsion form/cytotoxicity; Lycopene/emulsion form/permeation; Lycopene/emulsion form/rheology; Lycopene/emulsion form/thermal analyses; Phytocosmetics; Skin aging/treatment. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300579 |
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| Moreno,Andréia de Haro; Silva,Maria Fernanda Conz da; Salgado,Hérida Regina Nunes. |
| A stability study of azithromycin in ophthalmic preparations was developed by submission to different types of light, temperature and pH, using the biodiffusion assay (cylinder 3 x 3) for the quantifications. Bacillus subtilis, ATCC 9372, was used as test organism. The used concentration range was of 50 to 200 µg/mL. The study demonstrated that the drug suffered degradation when submitted to the ultraviolet light, germicide light, solar luminosity, acid solution, basic solution and hydrogen peroxide solution. The results were analyzed by the analysis of variance (ANOVA). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Azithromycin/stability study; Bioassay; Photodegradation; Ophthalmic preparations. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200005 |
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| Curbete,Mariane Machado; Salgado,Hérida Regina Nunes. |
| ABSTRACT Fusidic acid is an antibiotic steroid indicated for the treatment of infections caused by the genus Staphylococcus, including methicillin resistant Staphylococcus aureus strains, and other Gram-positive bacteria. In the present study, a stability-indicating reversed-phase liquid chromatography (RP-LC) method was developed and validated for the determination of fusidic acid in dermatological cream as an alternative to existing methods. Analyses were performed using a C18 column and guard column at room temperature, eluting with an isocratic mobile phase of acetonitrile and water (72:28, v/v), adjusted to pH 3.5 with acetic acid, pumped at a flow rate of 1.0 mL min-1, detection at 210 nm and 20 µL of injection volume. The forced degradation study... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusidic acid/stability-indicating/quality control/validation; Chromatography/reversed-phase.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000300447 |
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| Migliato,Ketylin Fernanda; Carvalho,Elisângela Simões de; Sacramento,Luis Vitor Silva do; Mello,João Carlos Palazzo de; Baby,André Rolim; Velasco,Maria Valéria Robles; Salgado,Hérida Regina Nunes. |
| A precise, accurate and low cost spectrophotometric method was developed and validated for routine determination of total polyphenols, as pyrogallic acid equivalents, from the percolated and lyophilized extract of Syzygium cumini (L.) Skeels fruits. Validation was assessed experimentally and data were rigorously treated by statistical analysis. Analytical parameters were: linearity, interval (range), precision and recovery/accuracy, limit of detection (LOD, μg mL-1) and limit of quantification (LOQ, μg mL-1). The visible spectrophotometric method presented linearity (r² = 0.9979 ± 0.0010) over the concentration range 0.25-7.5 μg mL-1 of standard pyrogallic acid, precision < 2.918171%, recovery/accuracy ranging from 96.228693 to 107.17701%, LOD = 0.21 μg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Total polyphenols; Syzygium cumini; Botanical extract; Analytical method validation; Spectrophotometry. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100015 |
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| Registros recuperados: 12 | |
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